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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
2015, Cilt 29, Sayı 1, Sayfa(lar) 007-010
[ Turkish ] [ Tam Metin ] [ PDF ]
Evaluation of Intra-Operative Culture and Antibiogram Results by Children Who Underwent Appendectomy
Ayşe SAĞMAK TARTAR1, Mehmet SARAÇ2, Affan DENK3, Tugay TARTAR4
1Cizre Devlet Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji, Şırnak, TÜRKİYE
2Fırat Üniversitesi Tıp Fakültesi Hastanesi, Çocuk Cerrahi Kliniği, Elazığ, TÜRKİYE
3Fırat Üniversitesi Tıp Fakültesi Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Elazığ, TÜRKİYE
4Cizre Devlet Hastanesi, Çocuk Cerrahi Kliniği, Şırnak, TÜRKİYE
Keywords: Appendectomy by children, culture, antibiotic susceptibility

Objective: One of the major causes of intra-abdominal infections is perforated appendicitis. The microbioloy findings obtainedby perforated appendicitis reflect microorganisms common in community- acquired intra-abdominal infections. In this study, it is aimed to determine regional resistance pattern in community- acquired intra-abdominal infections by examining intraoperative culture and antibiogram results gby children who underwent appendectomy.

Materials and Methods: 44 patients between the ages of 2-16 years underwent appendectomy with the diagnosis of appendicitis in clinical Pediatric Surgery between January 2013- 2014 were enrolled to the study. 1-10 mL peritoneal fluid or appendicular tissue taken from patients intraoperatively were cultured to blood agar and EMB agar plates. Antibiotic susceptibility of isolated bacteria was investigated with disk diffusion method according to CLSI suggestions direction. Results were considered as sensitive and resistant.

Results: Appendectomy was performed by 44 patients in our clinic during our study. Perforated appendicitis was found in 20 patients (45 %), acute appendicitis in 8 patients (18%), suppurative appendicitis in 16 patients (37 %) were identified. Reproduction were found on 33 (75%) in intraoperative cultures from patients. Reproduction were not found in 11 (25 %) patients. By 27 (82%) Escherichia coli, 2 (6%), Streptococcus spp. was isolated as effective pathogen. Enterobacter cloacae, Klebsiella spp., Enterococcus spp. and Micrococcus spp growth was observed on single case for each (3%). ESBL positivity was detected in 3 (11%) of E. coli strains. Ampisilin-sulbactam resistance by 61% (n= 17), quinolone resistance by 20.7% (n= 6) was found in isolated Gram-negative bacteria.

Conclusions: This study has acquired particular importance because it determines factor and resistance profile in community- acquired intra-abdominal infections. It also shed light on the empirical treatment of intra-abdominal infections. We cannot find enough work in our country about this subject and we need extensive research with a larger number of patients.

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