[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ] |
Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi |
2017, Cilt 31, Sayı 3, Sayfa(lar) 111-116 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Examining the Effects of Saxagliptin and Sitagliptin on the Cell Viability of Different Human Cancer Types |
Suat TEKİN1, Asiye BEYTUR1, Murat ÇAKIR2, Çiğdem TEKİN3, Süleyman SANDAL1 |
1İnönü Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Malatya, TÜRKİYE 2Bozok Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Yozgat, TÜRKİYE 3İnönü Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu, Malatya, TÜRKİYE |
Keywords: A2780, MCF-7, PC-3, LNCaP saxagliptin, sitagliptin |
Objective: Incretin hormones are classified in hypoglycemic gastrointestinal hormone group. Dipeptidyl peptidase-4 enzyme (DPP-4) inactivates incretins. DPP-4 inhibitors prevent the collapse of incretins and balance the increasing blood glucose. Saxagliptin and sitagliptin are the new members of this family (DPP-4 inhibitor). It was reported that the DPP-4 inhibitors have effects on blood glucose levels, and they also have antioxidant and anti-inflammatory effects. This study was conducted to investigate the anti-cancer properties of saxagliptin and sitagliptin, which are the members of DPP-4 inhibitor family.
Materials & Methods: The human ovary (A2780), human breast (MCF-7) and human prostate (PC-3 and LNCaP) cancer cell lines were used in the study. The changes occurring in the cell viability for 24 h in all cell lines in 1, 5, 25, 50 and 100 μg/mL of saxagliptin and sitagliptin 1, 5, 25, 50 and 100 μg/mL concentrations were determined with the 3-(4,5-dimethylimidazole-2-il)-2,5- diphenyltetrazolium bromide (MTT) assay method. According to the MTT assay results, the inhibiting concentration 50 (LogIC50) value was calculated in the Graphpad Prism 6 Program. Results: It was determined that the sitagliptin and saxagliptin that were incubated with cancer cells (A2780, MCF-7, PC-3 and LNCaP) for 24 h caused significant decreases in the viability of the cancer cells (P<0.05). Conclusion: The results of the present study show that the saxagliptin and sitagliptin, which were tested, have antitumor activity on A2780, MCF-7, PC-3 and LNCaP cells. |
[ Turkish ] [ Tam Metin ] [ PDF ] |
[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ] |