[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ]
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2022, Cilt 36, Sayı 2, Sayfa(lar) 134-139
[ Turkish ] [ Tam Metin ] [ PDF ]
Identification of Bacterial Pathogens from Nasal Cavity in Cattle with Pneumonia by MALDI-TOF MS
Pelin Fatoş POLAT DİNÇER1, Zeynep YERLİKAYA2, Burcu KARAGÜLLE2
1Dokuz Eylul University, Faculty of Veterinary Medicine, Department of Internal Medicine, İzmir, TÜRKİYE
2Fırat University, Faculty of Veterinary Medicine, Department of Microbiology, Elazığ, TÜRKİYE
Keywords: MALDI-MS, cattle, respiratory, pneumonia

The aim of our study was to show the etiological spectrum of pathogens in the nasal cavity of cattle with pneumonia using MALDI-TOF MS. The study material consisted of 64 dairy cows between the ages of 2 and 7 years, 39 calves aged between 30 and 85 days showing pneumonia symptoms and 20 healthy dairy cows and calves sampled from 24 farms in different districts of İzmir, Turkey. After clinical examination of all animals, nasal samples were taken using a swab with Charcoal Amies transport medium. The clinical examination revealed that the respiratory rate, heart rate, and rectal temperature were statistically higher in cases with pneumonia symptoms than in control cases (p<0.001). MALDI-TOF MS isolated 17 different bacteria from the dairy cow samples. The most frequently isolated bacterial pathogens were Escherichia coli (E. coli (32.8%), Staphylococcus lentus (S. lentus) (28.1%) and Staphylococcus sciuri (S. sciuri) (21.8%). Of the 11 different bacteria isolated from calves, the most frequent ones were E. coli (46.1%), S. lentus (41.0%), Staphylococcus xylosus (S. xylosus) (7.6%) and S. sciuri (7.6%). Culture and conventional PCR methods were used to determine the presence of Mycoplasma in the samples. In 29 of the samples taken from cattle and calves, Mycoplasma spp. M. bovis was isolated in 24. While S. sciuri (12.5%) and E. coli (12.5%) were detected the most among the factors causing mixed infection with M. bovis. While these findings reveal the spectrum of pneumonia agents in our region, they show that pathogens in the nasal cavity can be detected quickly and reliably with MALDI-TOF MS.

[ Turkish ] [ Tam Metin ] [ PDF ]
[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ]