In this study, the effects of 17-β estradiol (E2) and vitamin E on the plasma, glucose, lipid profile
and lipid peroxidation levels in ovariectomized and diabetic rats were investigated. In study, 70
female Wistar albino rats were randomly divided into 7 groups. Group 1: Control group, Group 2:
Ovariectomy + diabetes, Group 3: Ovariectomy + diabetes + E2, Group 4: Ovariectomy + diabetes
+ E2 + vitamin E, Group 5: Ovariectomy, Group 6: Ovariectomy + E2, and Group 7: Ovariectomy +
E2 + vitamin E. Sixty of rats were ovariectomized. After fifteen days from ovariectomy, to induce
diabetes, rats were injected with streptozotocin (STZ) intraperitonellly (I.P.) at dose of, 45 mg/kg
body weight. Following the formation of diabetes, E2 (40 µg/kg/day) was administered subcutan
(S.C.) to groups 3, 4, 6 and 7; additionally, vitamin E (100 mg/kg/day) was administered I.P. only to
groups 4 and 7 for a period 28 days. According to the findings of this study,due to the effect of
ovariectomy, glucose, total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), very low
density lipoprotein-cholesterol (VLDL-C), triglyceride (TG), and plasma and erythrocyte
malondialdehyde (MDA) levels were found to increased (p<0.001), but HDL-C (high density
lipoprotein-cholesterol) level was decreased (p<0.001), in group 5 when compared with group 1. In
spite of the negative scene of ovariectomy, in groups (groups 6, 7), glucose (p<0.01), TK
(p<0.05,p<0.001), LDL-K (p<0.001), VLDL-K (p<0.001), TG (p<0.001), plasma and eritrosit MDA
(group 7, p<0.001, p<0.01) levels reduced, but HDL-C level (p<0.001) was increased by
administration of E2 or E2 + vitamin E. Due to the effects of ovariectomy and diabetes, glucose, TC,
LDL-C, VLDL-C, TG, glycated hemoglobin (HbA1c), plasma and erythrocyte MDA levels were found
to increased (p<0.001), but HDL-C level was decreased (p<0.01) in group 2 when compared with
group 5. In spite of this negative effects of ovariectomy and diabetes, in groups (groups 3, 4),
glucose, TC, LDL-C, VLDL-C, TG, HbA1c (p<0.001), plasma and erythrocyte MDA (group 4,
p<0.001) levels reduced, but HDL-C level (p<0.001, p<0.01) was increased by administration of E2
or E2 + vitamin E. In conclusion, the risk of atherosclerosis resulting from increased lipid
peroxidation and impaired lipid profile in ovariectomized and diabetic rats may be reduced by
administrating E2 or E2 + vitamin E.
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