[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ]
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2009, Cilt 23, Sayı 1, Sayfa(lar) 021-028
[ Turkish ] [ Tam Metin ] [ PDF ]
Effects of Vitamin E and 17-β Estradiol on Plasma Lipid and Lipid Peroxidation Levels in Ovariectomized and Diabetic Rats
Mustafa ULAŞ, Mehmet ÇAY
Fırat Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Elazığ, TÜRKİYE
Keywords: Diabetes, Ovariectomy, Estradiol, Vitamin E and Lipid peroxidation

In this study, the effects of 17-β estradiol (E2) and vitamin E on the plasma, glucose, lipid profile and lipid peroxidation levels in ovariectomized and diabetic rats were investigated. In study, 70 female Wistar albino rats were randomly divided into 7 groups. Group 1: Control group, Group 2: Ovariectomy + diabetes, Group 3: Ovariectomy + diabetes + E2, Group 4: Ovariectomy + diabetes + E2 + vitamin E, Group 5: Ovariectomy, Group 6: Ovariectomy + E2, and Group 7: Ovariectomy + E2 + vitamin E. Sixty of rats were ovariectomized. After fifteen days from ovariectomy, to induce diabetes, rats were injected with streptozotocin (STZ) intraperitonellly (I.P.) at dose of, 45 mg/kg body weight. Following the formation of diabetes, E2 (40 µg/kg/day) was administered subcutan (S.C.) to groups 3, 4, 6 and 7; additionally, vitamin E (100 mg/kg/day) was administered I.P. only to groups 4 and 7 for a period 28 days. According to the findings of this study,due to the effect of ovariectomy, glucose, total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C), triglyceride (TG), and plasma and erythrocyte malondialdehyde (MDA) levels were found to increased (p<0.001), but HDL-C (high density lipoprotein-cholesterol) level was decreased (p<0.001), in group 5 when compared with group 1. In spite of the negative scene of ovariectomy, in groups (groups 6, 7), glucose (p<0.01), TK (p<0.05,p<0.001), LDL-K (p<0.001), VLDL-K (p<0.001), TG (p<0.001), plasma and eritrosit MDA (group 7, p<0.001, p<0.01) levels reduced, but HDL-C level (p<0.001) was increased by administration of E2 or E2 + vitamin E. Due to the effects of ovariectomy and diabetes, glucose, TC, LDL-C, VLDL-C, TG, glycated hemoglobin (HbA1c), plasma and erythrocyte MDA levels were found to increased (p<0.001), but HDL-C level was decreased (p<0.01) in group 2 when compared with group 5. In spite of this negative effects of ovariectomy and diabetes, in groups (groups 3, 4), glucose, TC, LDL-C, VLDL-C, TG, HbA1c (p<0.001), plasma and erythrocyte MDA (group 4, p<0.001) levels reduced, but HDL-C level (p<0.001, p<0.01) was increased by administration of E2 or E2 + vitamin E. In conclusion, the risk of atherosclerosis resulting from increased lipid peroxidation and impaired lipid profile in ovariectomized and diabetic rats may be reduced by administrating E2 or E2 + vitamin E.

[ Turkish ] [ Tam Metin ] [ PDF ]
[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ]