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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
2009, Cilt 23, Sayı 3, Sayfa(lar) 151-154
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Ghrelin Expression in Liver of the Toluene Inhaled Rats
Ufuk TAŞ1, Murat ÖGETÜRK2, Sedat MEYDAN3, Hilal IRMAK SAPMAZ2, Dürrin Özlem DABAK4, Tuncay KULOĞLU4, Mustafa SARSILMAZ2
1Gaziosmanpaşa Üniversitesi, Anatomi Anabilim Dalı, Tokat, TÜRKİYE
2Fırat Üniversitesi, Tıp Fakültesi, Anatomi Anabilim Dalı, Elazığ, TÜRKİYE
3Mustafa Kemal Üniversitesi Tayfur Ata Sökmen Tıp Fakültesi, Anatomi Anabilim Dalı, Hatay, TÜRKİYE
4Fırat Üniversitesi, Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, Elazığ, TÜRKİYE
Keywords: Toluene, ghrelin, liver, rat

Toluene is an aromatic hydrocarbon has addictive and toxic effect that is used widely in the industry. Ghrelin was a recently identified multifunctional hormone that is found in many organs, including the liver. This study aimed to determine ghrelin expression via immunohistochemical methods in rats that were experimentally administered toluene, a toxic agent for liver.

For this purpose, 14 adult male Wistar-albino rats were divided into two equal groups. Animals in group I were used as control group. The rats in group II were exposed toluene (3000 ppm/1hour/day) for four-weeks. Rats from both groups were decapitated 30 days after the operation. The liver tissues were removed and fixed in % 10 neutral formalin solution. Then tissue specimens were embedded in paraffin and sectioned (thickness, 5 µm). The sections were immunohistochemically stained using avidin-biotin-peroxidase method for determination ghrelin immunoreactivity.

Ghrelin immunoreactivity in the hepatocytes was determined as strong (+3) in the control group and low (+1) or no staining in the toluene inhaled group. No immunoreactivity was observed in negative control specimens. Ghrelin immunoreactivity was detected in sections of rat stomach used as a positive control.

The results obtained in our study indicate that toluene inhalation causes a decrease in ghrelin expression in the liver.


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