The aim of this study was to determine the changes of serum protein fractions of beef cattle vaccinated with foot and mouth disease vaccine and simultaneously applied with vitamin AD₃E and C. Fourty-eight clinically healty beef cattle, approximately aged one year, were used in this study. These animals were divided into four groups each in includes 12 animals (A, B, C and D). Inactivated foot and mouth disease (FMD) vaccine was only aplicated in group A, inactivated FMD vaccine and vitamin C on day 0 and 3 were injected in gruop B, inactivated FMD vaccine and vitamin AD₃E were injected in gruop C and inactivated FMD vaccine and vitamin AD₃E and C were injected in gruop D. Serum total protein levels were measured by a Biuret method, and serum total protein fractions measured by Laemmli methods. In conclusion, the means of gamma globulin levels in group C and D were significantly (p<0.001) increased compared with those of the other groups, and vitamin AD₃E and C application simultaneously with vaccination was determined that usefull effects on immune system.