The current study was undertaken to evaluate paraquat induced oxidative stress on caspase dependent apoptosis, cytochrom C levels and DNA laddering pattern in hepatocellular carcinoma cell line. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) cell viability and proliferation assay were performed different paraquat concentrations and time periods. 24h 10mM paraquat treatment was settled as IC50 for HepG2 cell line. Malondialdehyde, a marker of lipid peroxidation was estimated with TBA method. Caspase 3-9 were analyzed at 6., 12. and 24.h as apoptosis indicator with colorimetric assay. Cytochrom C levels were also estimated within the same time periods. DNA laddering assay performed to evaluate DNA fragmentation during apoptosis. The results indicated that there was no statistically significant difference after 6.h and 12.h but significant increase in 24.h for caspase 3. On the other hand statistically significant increase has been found after 12. and 24.h for caspase 9 compared to the control group. In all groups and time periods cytocrom C leves has been found at the same level. DNA fragmentation pattern has not been found in control and experiment groups. Paraquat induced oxidative stress were caused caspase activation. These observations suggested that oxidative stress caused by paraquat may be used as an in vitro model for caspase depended apoptosis pathways in HepG2 cell line.