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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2013, Cilt 27, Sayı 1, Sayfa(lar) 035-037
[ Turkish ] [ Tam Metin ] [ PDF ]
Detection of Bovine Ephemeral Fever Virus Infections by Polymerase Chain Reaction in Some Regions of Turkey in 2008
Şükrü TONBAK1, Engin BERBER1, Mehmet ÇABALAR2
1Fırat Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Elazığ, TÜRKİYE
2Harran Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Şanlıurfa, TÜRKİYE
Keywords: Bovine ephemeral fever virus, RT-PCR, cattle

Bovine ephemeral fever (BEF) is a viral disease of cattle which is characterized by hematological and biochemical changes. It can cause significant economic impact due to reduced milk production in dairy herds, and loss of condition in beef cattle. In the present study, a total of 110 whole blood and 120 sera were collected from cattle in 10 provinces located in Southeastern Anatolia, Eastern Anatolia, and Mediterranean region in 2008. After total RNA extraction of the samples, reverse transcriptase polymerase chain reaction (RT-PCR) was used to recover partial sequences of the BEFV glycoprotein gene. Glycoprotein gene was found in 20 samples by polymerase chain reaction by using specific primers in 2008.

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