Pyometra is an acute or chronic suppurative inflammation of the uterus, primarily observed in older animals. Delayed diagnosis and progression of the disease significantly increase mortality rates. Moreover, biochemical changes in queens with pyometra are generally ambiguous, and hematological abnormalities are mostly absent. In mild cases, no detectable changes may be observed. Queens are known for their meticulous grooming behavior, which can obscure clinical signs. Additionally, as pyometra is more commonly associated with older animals, suspicion of the condition in juvenile individuals is often overlooked. This study investigates cases of pyometra diagnosed in juvenile queens, particularly in those that have never given birth. The animal material of the study comprised 20 juvenile queens in total, including 10 healthy juvenile queens and 10 juvenile queens diagnosed with pyometra. Hemogram and biochemical parameters were evaluated from blood samples collected from the animals. According to the complete blood count, the white blood cell (WBC), hematocrit (HCT), and mean corpuscular volume (MCV) values of the pyometra queens were found to be increased, while the monocyte (MON) value was decreased (p<0.05). The alkaline phosphatase (ALP) levels in the pyometra queens were found to be decreased (p<0.05). In conclusion, while pyometra is generally considered a disease of middle-aged and older queens, it can also occur in young queens. Early diagnosis and ovariohysterectomy are effective in restoring health in affected queens. Further studies involving larger sample sizes and diverse breeds of young queens with pyometra are recommended to provide more comprehensive and meaningful insights.