In this study, it was aimed to evaluate the pregnancy rates obtained from ewes inseminated by intracervical artificial insemination with frozen semen obtained from rams treated with autologous PRP intraepididymally and frozen semen containing homologous PRP in semen diluents. For this purpose, 75 Akkaraman ewes were synchronized using intravaginal sponges and divided into three groups, each consisting of 25 animals. Group 1: inseminated with frozen semen without PRP (Control group), Group 2: inseminated with frozen semen obtained by intraepididymal PRP application to rams (In vivo PRP group), Group 3: inseminated with frozen semen containing 5% PRP in its diluent (In vitro PRP group). Reproductive parameters including gestation period, lambing rate, multiple birth rate, female and male lamb ratios and lamb birth weight were recorded for each group. Although the pregnancy rate in the In vitro PRP group was higher than in the control and in vivo PRP groups, it was not statistically significant. The differences observed between all groups in terms of gestation periods were not significant. All sheep in the Control and In vivo PRP groups gave single births, while one sheep in the In vitro PRP group gave twin births. There was no significant difference between the groups in terms of multiple birth rates. The differences observed between the groups in terms of female and male lamb ratios and lamb birth weights were not statistically significant. In conclusion, although in vivo PRP has no effect on reproductive parameters, intracervical insemination with frozen-thawed semen containing 5% in vitro PRP is recommended based on the slight increase observed in reproductive parameters.