In the study, the effects of artichoke on pyruvate kinase (PK) activity in the heart tissue of rats treated with nitrosomorpholine (NMOR) were evaluated in vitro and in silico. 54 Wistar Albino male rats were divided into 6 groups, 7 rats in the control and artichoke groups and 10 rats in the other groups. No treatment was performed on the rats in the control group. The other groups were administered artichoke (1.5 g/kg/day), NMOR10 (10 mg/kg/day), NMOR30 (30 mg/kg/day), NMOR10 (10 mg/kg/day)+artichoke and NMOR30 (30 mg/kg/day)+artichoke, respectively. Both substances were administered by gavage at the indicated doses for 14 days. At the end of the study, PK activities were determined in the heart tissue. There was no statistically significant difference in PK activity between control group and artichoke treated group. When compared with the control group, heart tissue PK activity was found to be lower in the NMOR10 and NMOR30 groups, where NMOR was administered at doses of 10 and 30 mg/kg (p=0.013 and p<0.001, respectively). Heart tissue PK activities were observed to be higher in the NMOR10+Artichoke group compared with the NMOR10 group, and in the NMOR30+Artichoke group compared with the NMOR30 group (p=0.01 and p=0.019, respectively), and it was observed that the means of these groups approached the means of the control group (p>0.05). It was calculated that Cynarin would inhibit at 12.42 nanomolar, Luteolin at 42.90 micromolar, Cynaroside at 69.62 micromolar, and Scolymoside at 1.47 millimolar levels. When the interaction mode, the types of bonds it interacts with, and docking scores were evaluated together, it was found that Cynarin and Luteolin would be more effective, respectively, while Cynaroside and Scolymoside would be relatively less effective, respectively, for PK inhibition. The results suggest that NMOR affects PK activity in cardiac tissue and may play a role in its pathogenesis. In addition, it has been observed that the artichoke supplementation has a regulatory effect in the heart of NMOR treated rats.