This study aimed to investigate the effects of some sedatives and hypnotics such as Acepromazine, Haloperidol, Detomidine, Medetomidine and Xylazine, on tear quantity in rabbits. The study material comprised four male and four female New Zealand white rabbits. Before injection, tear quantity was measured in each rabbit using Schirmer 1 tear strips (T-0). Then, Acepromazine 3.3 mg/kg, Haloperidol 5 mg/kg, Detomidine 0.30 mg/kg, Medetomidine 0.25 mg/kg and Xylazine 5 mg/kg were administered intramuscularly to the quadriceps femoris muscle in each group. Following the injection, tear volume was measured at 15 (T-1), 30 (T-2), 45 (T-3), and 60 minutes (T-4), and 24 hours (T-5) after sedation. Statistically significant decreases in tear volume were found in the Xylazine (p=0.024) and Detomidine (p=0.001) groups at 15 minutes following the injection compared to the pre-sedation times. On the other hand, increases were observed in tear volumes in measurements taken 15 minutes after the Haloperidol (p=0.002) and Acepromazine (p<0.0001) injection. In the Xylazine, Detomidine, and Medetomidine groups, a slight increase was observed 30, 45, and 60 minutes after injection, while measurements taken 24 hours later were found to be close to the initial value. As a result of this study, it was concluded that the reductions in tear volume in rabbits may serve as a reference during future short-term surgical procedures performed with the sedatives and hypnotics used in this study in veterinary medicine, and that corneal xerosis and related diseases such as keratitis, keratoconjunctivitis, corneal ulcers, and eye loss due to decreased tear production can be prevented.