Hepatozoon species are known important vector-borne pathogens and these species may infect various hosts. Hepatozoon species, Hepatozoon felis, H. canis and H. silvestris, infect domestic and wild cats, and these species may cause mild to severe clinical manifestations in cats. There have been numerous studies conducted to the determination of Hepatozoon species in cats from different countries. However, there are paucity of data on presence and prevalence of Hepatozoon species among cat population in Türkiye. In this study, 320 gDNA obtained from cat blood samples collected from Sivas, Ankara, Malatya, Denizli, and İstanbul provinces were investigated for Hepatozoon species using genus-specific PCR assay. All PCR positive samples were sent to DNA sequences analyses. In the current work, Hepatozoon spp. were detected in three (two samples in Sivas and one sample in Malatya) of 320 cat samples (0.93%, 3/320; 95% CI 0.19-2.72). According to the results of DNA sequence, all positive samples were identified as H. felis. However, H. canis and H. silvestris was not detected in cat samples. Determined consensus sequences were deposited in the GenBank with accession numbers: PV639385-PV639387. BLASTn analyses revealed that 97.72–100% nucleotide identities were determined between our H. felis isolates and H. felis isolates present in GenBank. The results of the study showed that H. felis is circulated among owned cats in Türkiye. Considering the H. felis may cause infection in cats, necessary precaution should be taken to protect stray or owned cats health by veterinarians and cat owners.