The present study aims to investigate the histopathological effects of chemical castration performed by intratesticular administration of high-osmolarity solutions on the testes. The study included 56 male Wistar rats, divided into seven groups of eight animals each. Group 1: Control; Group 2: Intratesticular 0.9% saline solution (Sf); Group 3: Intratesticular 0.9% saline solution with 10% calcium chloride (CaCl₂); Group 4: Intratesticular 20% mannitol (Man); Group 5: Intratesticular 20% mannitol with 10% CaCl₂; Group 6: Intratesticular 20% dextrose (Dex); Group 7: Intratesticular 20% dextrose with 10% CaCl₂ injection group. Following a period of sixty days, histopathological changes and cell apoptosis were evaluated. When compared to the Sf and control groups, the high-osmolarity, Deks, and Man groups used for chemical castration were found to be more effective in terms of histological and apoptotic cell scores. Groups subjected to treatment with CaCl₂ combinations (0.9% Sf + 10% CaCl₂, 20% Mna + 10% CaCl₂, 20% Dex + 10% CaCl₂) exhibited superior histopathological efficacy in comparison to all other experimental groups.