The involvement of eyelids by dermatophytes is unfrequent in horses. In this article, characteristics, diagnosis, clinical signs and therapy course of mycotic blepharitis due to Trichophyton equinum in a dressage horse were described. Trichophyton equinum was isolated from broken eyelashes and scales of annular lesions of eyelids. The horse was successfully treated with topical terbinafine 1% cream resulting in clinical and mycological cure.