The aim of this study was to determine the effect(s) of long-time anesthesia generated by isoflurane, sevoflurane, propofol-fentanyl, medetomidine-midozolam-ketamine or xylazine-ketamine combinations on hemodynamic parameters, serum cardiac troponin-I, and C-reactive protein concentrations in rabbits. The study was performed on 30 New Zeland rabbits. Rabbits were divided into 5 groups. In group 1, induction was performed with isoflurane, and anesthesia lasted for four hours. In group 2, the same procedure in group 1 was applied using sevoflurane instead. In group 3, midazolam, medetomidine and ketamine were administered intramuscularly. Anaesthesia protocols lasted for four hours with additional injections. In group 4, anaesthesia was induced with fentanyl and propofol and maintained with fentanyl and propofol) for four hours. In group 5, xylazine were used for premedication. General anesthesia was performed with ketamine. Four hour anesthesia was maintained. The reflexes between induction and recovery time were evaluated. Haemodynamic and respiratory variables were recorded. Blood samples were analyzed for biochemical parameters (CRP and cTn-I).

While group 5 or group 3 protocols provided analgesia for major surgical approaches, group 1 and group 2 anaesthetics were enough for minor surgical approaches. In group 2 and group 3, heart rates decreased compared to pre-induction. When we measured the CRP and cTn-I serum levels before, during and after 6, 12 and 24 hours of induction, no significant change in values observed in between the groups. In conclusion, cardiac troponins could be used to detect acute myocardial damage associated with anesthesia both experimental and clinical studies.