This study was carried out in order to reveal the presence and prevalence of hemotropic Mycoplasma spp. in goats in Antalya region by nested PCR. For this purpose, a total of 157 blood samples were collected from the 3 focus of the Antalya province (Akseki, Manavgat and Serik). The animals used in the study were also examined for presence of ticks. Total genomic DNAs were isolated from these samples, and DNA samples were obtained from hemotropic Mycoplasma spp. the presence of the 16S rRNA gene region was amplified by nested PCR. 157 of the samples examined, 35 (22.3%) were Mycoplasma spp. positivity was detected. Sequence analysis of the selected among the positive samples, was found to be 100% similar to the result of Mycoplasma ovis. In this study, nested PCR of hemotropic Mycoplasma spp. presence and epidemiological data of the agent were obtained.