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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi |
2018, Cilt 32, Sayı 2, Sayfa(lar) 087-091 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Investigation of the Effects of N- (p-amylcinnamoyl) Anthranilic Acid (ACA) on Human Over and Prostate Cancer Cell Viability |
Murat ÇAKIR1, Suat TEKİN2, Asiye BEYTUR2, Süleyman SANDAL2 |
1Yozgat Bozok Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Yozgat, TÜRKİYE 2İnönü Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Malatya, TÜRKİYE |
Keywords: N-(p-amylcinnamoyl) antranilik asit (ACA), transient receptor potential melastatin-2 (TRPM2) channels, phospholipase A2, cancer |
Objective: Prostate and ovarian cancers are among the most common types of cancer in Turkey. N- (p-amylcinnamoyl) anthranilic acid (ACA) is an inhibitor of both transient receptor potential melastatin-2 (TRPM2) channels and phospholipase A2 (PLA2). Recent studies have reported that TRPM2 channels and PLA2 are associated with cancer. The aim of the study is to investigate anti-cancer mechanism of ACA in ovarian (A2780) and prostate (PC-3 and LNCaP) cancer cells.
Materials and Methods: A2780, PC-3 and LNCaP cell lines were used in the study. All cell lines were incubated with ACA at concentrations of 1 μM, 5 μM, 25 μM, 50 μM and 100 μM for 24 h. Changes in cell viability were examined by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) method. The inhibitory logarithmic concentration 50 (LogIC50) values were calculated according to MTT assay results. Results: There was a significant decrease in survival of cancer cells (A2780, PC-3 and LNCaP) incubated with ACA for 24 hours (P<0.05). All concentrations of ACA added to the culture medium caused a decrease in A2780 cell viability (P<0.05), In PC-3 and LNCaP cells, all concentrations of ACA except 1 μM were found to reduce cell viability for 24 hours (P<0.05). Furthermore, when LogIC50 values were examined, it was determined that ACA showed the strongest cytotoxic activity on LNCaP cells at lower concentration. Conclusion: ACA was found to have cytotoxic activity on the A2780, PC-3 and LNCaP cells. These findings suggest that ACA has antitumor properties. |
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