Bluetongue (BT) is a non-contagious animal disease spread by blood-feeding midges (Culicoides spp.) reported in domestic and wild ruminants in many parts of the world, including Türkiye. Bluetongue virus (BTV), the causative agent, belongs to the genus Orbivirus in the family Reoviridae in virus taxonomy. In this study, we aimed to investigate the seroprevalence of BT in healthy sheep in the provinces of Elazığ and Malatya in the Eastern Anatolian region of Türkiye, using a competitive enzyme-linked immunosorbent assay (cELISA; IDEXX, USA) designed for detection antibody against the virion protein 7 (VP7) of BTV. For this purpose, a total of 186 healthy sheep (Elazığ, n:100; Malatya, n:86) from five different locations in each province were included in the study, and serum samples were separated from their blood samples collected from their jugular vein were used in cELISA. According to the cELISA results in this study, the BT seropositivity of sheep in Elazığ and Malatya was 10% (10/100) and 0% (0/86), respectively, while the overall seroprevalence was 5.38% for both provinces. Information on the epidemiology of BTV infections in Elazığ and Malatya provinces is limited. Although this is a small-scale study, the current data obtained will contribute to the epidemiology of BTV in Türkiye, but more detailed studies are needed in this region.