The skin flora is a community of microorganisms that prevent pathogens from settling and growing on the skin and that live by grouping together without harming the organism. Populations of the microorganisms in the body differ according to the anatomical regions, however, there may be a varying population and flora in different individuals for the same anatomical regions. In this study, it was aimed to reveal the bacterial and mycotic agents in the skin flora of healthy cats and to investigate whether the flora differs according to the anatomical regions. The material of the study consisted of 20 cats, had no history of itching and severe hair loss in the anamnesis, and who were found to have no lesions, dandruff, hair loss and itching on the skin in their routine clinical examinations, and their hair was shiny and normal. The skin flora of the cats was investigated for mycotic and bacterial flora by taking 260 swab samples from different body parts. The bacterial agents isolated from the skin flora of healthy cats were identified as A. lwoffii, B. subtilis, B. zoohelcum, E. faecalis, E. faecium, K. rhizophila, K. rosea, K. varians, L. boronitolerans, M. luteus, N. zoodegmatis, P. multocida, P. luteola, P. putida, P. oryzihabitans, S. paucimobilis, S. epidermidis, S. capitis, S. felis, S. haemolyticus, S. hominis, S. simulans, S. xylosus, E. coli, and the mycotic agents were A. fumigatus, A. niger, Bipolaris spp, Malessezia spp, and Penicillium spp. As a result; It was determined that gram (+) bacteria were dominant in the skin flora of healthy cats, and the flora did not differ according to the anatomical regions, but bacterial and mycotic growth was more intense in some regions (nostril, oral cavity, vulva/prepisium).