Parasitic diseases occupy a very important place in human and animal health worldwide. Reducing the parasite load is a very important goal in the control and prevention of parasitic infections, but it cannot be applied alone. Therefore, environmental and ecological changes need to be implemented to reduce not only the parasite load but also the risk of parasite transmission. Vaccines are the most effective, safe and sustainable methods, and in this regard, they are also considered as alternative solutions to control parasitic diseases and are useful in reducing dependence on pharmacological drugs and the use of pesticides. DNA vaccination is a new technological method that uses genetically engineered DNA to generate an immunological response. An important strategy to achieve this goal is to use DNA plasmids with encoded antigens on them. This antigen-encoding DNA plasmid is capable of inducing the humoral and cellular immune response against parasites, bacteria and disease-producing viruses. DNA vaccines are a type of subunit vaccine currently in development. In this review, detailed information about the production and mechanism of action of DNA vaccines is given, and information about DNA vaccine studies against parasitic diseases is presented.