In this study, it was aimed to determine the levels of some oxidative stress and biochemical parameters, and to investigate the effects of the mode of delivery on these parameters in the mother cow, the umbilical cord at the time of birth, and in calves born before and after receiving colostrum, and on different days. 20 cows and calves were used in the study. Cows were divided into normal, dystocia, premature and cesarean sections. Blood samples were taken from the vena jugularis just before calving, from the umbilical cord immediately after calving, and from the calves at the time of calving, after colostrum administration, and on the 15th, 30th, 45th, and 60th days after calving from cows in all groups. Malondialdehyde (MDA), glutathione, glutathione peroxidase (GSH-PX), catalase levels of oxidative stress parameters in the samples were determined spectrophotometrically, total protein, urea, triglyceride, cholesterol, albumin, glucose, lactate dehydrogenase (LDH), triiodothyronine (T3), thyroxine (T4) levels and non-esterified fatty acids (NEFA) were determined by autoanalyzer. Dystocia, umbilical cord blood MDA levels of cesarean section groups were found to be higher than the values in maternal blood. When the umbilical cord blood MDA levels were compared, it was determined that there was an increase in the levels in the cesarean section group. MDA levels of calves in the post-colostrum strength, premature and cesarean section groups were found to be higher than the values of the calves in the normal birth group. The maternal blood GSH-Px levels in the cesarean section group were lower than the levels in the umbilical cord. The umbilical cord blood albumin, glucose, cholesterol, LDH and total protein levels of the premature and cesarean section groups were lower than the maternal blood values. When compared to the values in maternal blood, umbilical cord blood T3 levels were found to increase in premature and cesarean section groups, and umbilical cord T4 levels in the premature group were decreased. While umbilical cord serum urea levels were found to be higher in the premature group compared to the values in the mother, it was observed that umbilical cord blood NEFA levels decreased in the dystocia, premature and cesarean section groups. As a result, it was determined that the mode of delivery caused an increase in oxidative stress in the umbilical cord blood.