Due to hepatocellular damage, NH₃ level is increased and levels of urea cycle enzymes are reduced. NH₃ causes increase in the synthesis of mitochondrial carbamoyl phosphate. After diffusing into cytosole, carbamoyl phosphate is directed to pyrimidine synthesis by cytosolic carbamoyl phosphate synthase and increase in orotic acid production is observed. Objective of the present study was to evaluate orotic acid levels in experimental cirrhosis induced by CCl₄. Total of 36 rats (control: 17, experimental group: 19) were used in the study. Experimental cirrhosis was induced by CCl₄. Total protein, albumin, AST, ALT, ALP and LDH levels in serum were determined by an autoanalyzer whereas orotic acid levels were determined with HPLC. There was no significant changes in total protein levels whereas albumin levels were reduced in both group of rats. AST, ALT, ALP, LDH and orotic acid levels were found to be significantly increased in cirrhotic rats compared to control ones (p<0.005). There was a statistically significant increase in orotic acid levels in rats with experimental cirrhosis. Therefore, orotic acid level, in addition to other liver function parameters such as AST, ALT, ALP, LDH may be routinely determined so that more useful and meaningful results can be obtained about pathogenesis of liver diseases especially cirrhosis.