Hepatitis E virus (HEV) infection is sporadically encountered in developed countries. To date, whereas it has been found in developing countries as frequently as other infections transmitted by faecal-oral way. Hepatitis E prevalence was not known in Elazığ region. The aim of this study was to determine Hepatitis E prevalence, which was predicted to be high, and to exclude some important criteria in transmission of this infection. For this purpose, questionnaires were filled by interviewing face to face 707 volunteer healthy individuals aged 0 to 70 years. In the sera separated from blood samples obtained, anti-HEV was screened by using total macro-ELISA method (Abbott HEV kits). In general, anti-HEV seropositivity was detected in 82 out of 707 (11.6 %) between 0 and 70 years age. Anti-HEV positivity was found 4.2% in 1-9 years, 7.1% in 20-29 years, 10.3% in 30-39 years, 20.0% in 40-49 years and 26.3% in 50-59 years. While there was no difference wtih regard to anti-HEV positivity between sexes, it was obserwed that anti-HEV positivity decreased with education of individuals. Anti-HEV positivity was found in 12 out of 57(21.1%) individuals coming from rural areas and in 70 out of 650 individuals living in urban areas (p<0.05). Anti-HEV positivity was detected 24.0 % (154/37) in people living in traditional detached cottages made of soil and 8.1 % (553/ 45) in people lining in apartment flats (p< 0.05). On the other hand, anti-HEV positivity was found in 10 out of 127 pregnant women (7.8%). As a result, we report that the Elazığ region is amongst areas having high levels of anti-HEV seropositivity in Turkey. It was also observed that this ratio was increased in correlation with age and high in people living in rural areas and traditional detached cottages. Additionally, it was high in people having low level of education.