The aim of this study was to report the clinical, pathological and microbiological findings in the sheep with listeriosis. Twenty-five sheep that had neurological symptoms and mortality constitued the material of the study. The clinically anorexia, abortus, conjuctivitis, depression and neurological signs were determined in the diseased animals. The blood, feed, milk, and feces samples were taken from the sick sheep. The biochemical paramaters were between the reference values for sheep. The necropsie was carried out in three sheep, and PCR was applied to the samples of brain and spinal cord. The histopatological changes were seen especially in the brain, pons, medulla oblangata and grey matter. Listeria spp. suspected isolates, as indicated by black colonies on the Listeria Isolation Medium (Oxford formulation) agar plates were obtained from three sheep brain samples. In the analysis of culture positive samples by species-specific PCR, all isolates were identified as L. monocytogenes.