In this study, it was aimed to determine histological structure and histochemical features of Harderian gland of partridge (Alectoris chukar). The Harderian glands of 4 adult partridge were taken after euthanasia by cervical dislocation method. As a result of the applied histochemical methods, it was observed that carboxylated, O-sulfated esters and sulfated mucosubstances gave strong reaction in the corpus glandula epithelial cells. While acidic, carboxylated and neutral mucosubstance were moderate in the primary and central. Mucosubstance content was acidic character in the central canal mucus cells.