Irradiating biological material leads to a rapid increase in ROS, generated primarily due to the ionizing of water molecules. ROS are highly reactive and could diffuse to vital cellular targets like DNA, proteins and membrane, thus leading to cell death
4,25. Cellular and tissue resistance against ionizing radiation depends on many endogenous parameters, including antioxidant systems and their capacity for adaptive response
26. A number of studies have revealed that irradiation caused oxidative damage by causing an increase in MDA levels and a decrease in the levels of the antioxidants such as SOD, GSH, and GSH-Px depending on time and dosage
10,26-31. In this study, MDA levels in the liver, kidney, and brain increased significantly as a result of γ-radiation; whereas, a significant decrease was detected in the GSH-Px activity in these tissues and also in the SOD activity and GSH levels in the liver. Additionally, as a result of γ-radiation, significant decreases in GSH-Px activity were determined in the heart, spleen and ovarium tissues; whereas, MDA had an insignificant increase. The reduction in the antioxidant enzymes is associated with the depletion of enzymes during oxidative stress that occurred as a result of irradiation
10,26,28. Differently from studies indicating a decrease in GSH in the kidney and ovarium tissues
29,32,33, a significant increase was observed in GSH levels in these tissues as a result of γ-radiation in the present study. Also in a study conducted on the acute ovarian toxicity induced by the whole body irradiation, it has been reported that irradiation does not cause a change in the MDA level in the ovarium tissue and causes an increase in GSH-Px and CAT activities
34. Kandemir et al.
35, suggested that the activation of antioxidant defenses, in which the actual production of oxyradicals should decrease, is a preparative mechanism against oxidative stress caused by stress situations.
Irradiation causes oxidative damage in more than one tissue and although the cells have both enzymatic and non-enzymatic antioxidant systems to scavenge ROS formed as a result of irradiation, the antioxidant systems fall behind as mentioned above. In order to prevent the oxidative stress caused by irradiation, natural exogen antioxidants may be used. Propolis is the generic name for a complex resinous mixture collected by honey bees from the buds and exudates of various plants. The flavonoids in propolis are powerful antioxidants and capable of scavenging free radicals and thereby protecting the cell membrane against lipid peroxidation13,31,36. Antioxidant activity of flavonoids is based on ability of direct free radicals scavenging or stabilizing the reactive oxygen species (ROS) by interacting with the reactive compound of the radical. Due to the high reactivity of the phenolic hydroxyl substituents of the flavonoids, radicals are made inactive2. In this study, the MDA levels increased in the liver as a result of irradiation decreased significantly with the propolis addition; however, the MDA levels in the kidney and brain tissues were not affected by the addition of propolis. The decreases in the MDA levels by the propolis addition in heart, spleen, and ovarium tissues together with the liver tissues compared to the irradiation group may be associated with the antioxidant and free radical scavenging capability of propolis. In numerous studies, it has been determined that the addition of propolis decreases the MDA levels increasing in the plasma31, lens36, skin14, small intestine37 occurring as a result of irradiation and increased the decreased antioxidant levels such as SOD36, and GSH31,37. Being an active phenolic compound of propolis extract, caffeic acid phenethyl ester (CAPE) blocks ROS production13,36. Also in various studies investigating the effect of CAPE on radiation, similar results have been obtained about the oxidant and antioxidant parameters in the tissues such as liver4, intestine38, heart26, and lens36. Moreover, it has been reported that propolis is more effective than CAPE in preventing the oxidative damage occurring as a result of irradiation36. In contrast with other studies, the fact that the addition of propolis decreased the GSH-Px activity from antioxidant enzymes in all the tissues, SOD activity in the liver and heart tissue, GSH level in the kidney compared to the control and/or irradiation groups may be associated with the fact that propolis decreased the need for the antioxidants due to its antioxidant and free radical scavenging properties.
GSH is one of the most important anti-oxidant molecules. Propolis has been determined to reverse the consumption of glutathione, which is synthesized in the liver and has radical scavenging activity39. Also in this study, it was determined that while due to the addition of propolis, GSH levels, which decreased in liver as a result of irradiation, returned to the normal level, the GSH levels in the spleen and brain increased compared to the control group.
Radiation causes oxidative stress in more than one tissue and often the antioxidants run out. When the MDA levels were examined, it was observed that propolis decreased the oxidative stress in the tissues other than kidney and brain in the irradiated rats due to its antioxidant and free radical scavenging property. As propolis decreases the formation of lipid peroxidation in the irradiated rats, there is a need for conducting further studies on high doses of propolis by which the oxidative damage in the kidney and brain is prevented.