Taenia multiceps' adult form lives in the small intestines of carnivores such as dogs, jackals, and foxes. However, Coenurus cerebralis larvae settle in the central nervous system and spinal cord of sheep, goats, cattle, buffaloes, camels, and, rarely, humans causing coenurosis. The study aimed to assess the diagnostic efficacy of C. cerebralis cyst fluid antigen in sheep with coenurosis using the Western blot (WB) method. To achieve this, an antigen was prepared from the cyst fluid obtained from a C. cerebralis cyst. The proteins were separated using SDS-PAGE and transferred to a nitrocellulose membrane for WB. In the WB, 40 sheep sera infected with C. cerebralis and 40 newborn lamb sera were used as negative controls. Additionally, 20 hydatid cysts and 20 sheep sera infected with C. tenuicollis were tested to determine cross-reactions. The SDS-PAGE separation of cyst fluid antigen revealed eight bands with molecular weights of 12, 24, 34, 38, 50, 75, 106, and 180 kDa. After WB processing of sera, two bands with molecular weights of 24 and 68 kDa were observed. The samples that showed both bands were considered positive, while those with only the 68 kDa band were considered negative. Based on this evaluation, 17 out of 40 sheep sera that were definitively positive for C. cerebralis (42.5%) were found to be positive. However, all 40 sera that were definitively negative tested negative (100%). Out of the 20 hydatid cyst positive sera that were processed to determine cross-reactions, 9 (45%) gave positive responses, while 3 (15%) of the 20 C. tenuicollis positive sera gave positive responses. The data obtained in this study indicate that the use of crude cyst fluid antigen in WB for the diagnosis of C. cerebralis did not achieve sufficient diagnostic accuracy. Therefore, the development of diagnostic methods based on recombinant antigen would be useful.