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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2015, Cilt 29, Sayı 1, Sayfa(lar) 031-036
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Effects of Ceftiofur on Conctractions of Uterine Isolated From Non-Pregnant Cows
Nevzat SAAT, Halis ÖCAL
Fırat Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Elazığ, TÜRKİYE
Keywords: Ceftiofur, myometrium, contraction, cow

This study investigated the effects of ceftiofur, a third-generation cephalosporin on spontaneous contractility of the myometrium isolated from non-pregnant cows and heifer uterus.

Myometrial strips were isolated from cows and heifers that were at follicular phase and suspended in a 5 mL jacketed organ bath filled with Krebs solution at 38°C (pH 7.4) continuously bubbled with 95% oxygen and 5% carbon dioxide. After manifestation of the spontaneous contractions during equilibration period of 90 minutes, isometric contractions were recorded by using isometric force displacement transducer and initial 10 minute was considered as control period. Then, the test substances ceftiofur (500 μM, cumulative 1000 μM and 2000 μM, n=16) were added to the tissue bath and evaluated by 10-min intervals. The effects of ceftiofur on amplitude (mg), frequency and area under the contractile curve (AUC) of spontaneous contractions were evaluated by 10 min intervals. Data were statistically analyzed by using the Wilcoxon Signed Ranks Test and Pearson's Correlation Test.

It was determined that all tested doses of ceftiofur reduced the frequency of contractions (P<0.05), and did not affect the amplitude (P>0.05). After 500 μM and cumulative 1000 μM ceftiofur, the increase in the AUC found to be statistically insignificant (P>0.05). But after addition of cumulative 2000 μM ceftiofur the increase in the AUC found to be statistically significant (P<0.05).

In this in vitro study, it was found that all doses of ceftiofur reduced the frequency of contractions, did not affect the amplitude and increased AUC at high doses. It was concluded that considering the effects of the agent observed in this study could be important during their clinical use at pregnancy and postpartum period.


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