In the study, 40 female Wistar Albino rats were used to investigate the healing effects of amniotic fluid on corneal alkali burns. Rats were randomly divided into 4 groups of an equal numbers of rats. The groups were assigned as follows: The control group; the group “AF”, in which the rats were applied with bovine amniotic fluid for 20 days; the group “AB+SF”, in which an alkali burn was formed in the right eyes of the rats and saline was applied for 20 days; and the group “AB+AF”, in which an alkali burn was formed in the right eyes of the rats and amniotic fluid was applied for 20 days. At the end of the 20th day of the experiment, malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity were determined in the corneal tissue of the animals euthanized. The MDA levels and the GSH-Px activity in the corneal tissue were found to be increased significantly in the “AB+SF” group compared to the control group. When the MDA levels in the corneal tissue were compared between the “AB+AF” and the “AB+SF” groups, a statistically significant decrease was determined and the MDA levels approached to those obtained in the control group. The GSH levels in the corneal tissue were lower in the “AB+SF” group compared to the control group; the difference between these two groups was found to be significant. When the GSH levels in the corneal tissue were compared between the “AB+AF” and the “AB+SF” groups, a statistically significant increase was determined and the values approached the control group. In conclusion, oxidative stress may play a role in corneal alkali burns, and amniotic fluid may be effective in improving it.