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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2021, Cilt 35, Sayı 2, Sayfa(lar) 109-113
[ Turkish ] [ Tam Metin ] [ PDF ]
Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8-Hydroxy-2-Deoxyguanosine Expressions in Lambs with White Muscle Disease
Emin KARAKURT1, Özhan KARATAŞ2, Serpil DAĞ1, Enver BEYTUT1, Ali Sefa MENDİL3, Hilmi NUHOĞLU1, Ayfer YILDIZ1
1Kafkas University, Faculty of Veterinary Medicine, Department of Pathology, Kars, TURKEY
2Cumhuriyet University, Faculty of Veterinary Medicine, Department of Pathology, Sivas, TURKEY
3Erciyes University, Health Sciences Institute, Kayseri, TURKEY
Keywords: 4-HNE, 8-OHdG, dityrosine, lambs, white muscle disease

The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipid peroxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2-nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs with White Muscle Disease (WMD). The material of this study consisted of tissue samples taken from 20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routine histopathological diagnosis between 2012 and 2019. In macroscopic examinations, chalky-white large necrotic areas on the epicardial and especially on the endocardial surfaces of the right and left ventricular walls were observed. In microscopic examinations, it was observed that the degenerated muscle fibers in the heart were homogeneously pink in color, lost their cytoplasmic citration, swelled and their nuclei were pyknotic. Calcifications in necrotic and degenerated areas were detected. All white muscle disease cases were immune positive for 4-HNE, DT and 8-OHdG expressions in heart tissues. A significant difference (P<0.05) was detected between the groups in terms of immunoreactivity to 4-HNE, DT and 8-OHdG in immunohistochemical and immunofluorescence staining findings. In conclusion, our study showed that formation of ROS that is important in pathogenesis of WMD causes not only lipid peroxidation, but also protein modification and DNA damage.

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