The aim of the present study was to determine serum iron concentrations, total iron-binding capacity (TIBC), and albumin concentrations, and to examine the potential use of these markers to predict the severity of systemic inflammation in dogs with parvoviral enteritis by comparing these parameters with serum C- reactive protein (CRP). Eighteen dogs with parvoviral enteritis (PVE) and 16 clinically healthy dogs (Control) were included in this study. Serum CRP concentration was significantly higher (P<0.001) in the PVE group (18.50±7.50 mg/dL) compared to the Control group (0.50±0.14 mg/dL). While there was no significant difference in serum TIBC (P= 0.210) between groups, it was found that serum iron (P<0.001) and albumin (P= 0.005) concentrations decreased significantly in the PVE group compared to the Control group. Serum iron concentration of ≤73 μg/dL (AUC= 0.979, P< 0.001) and a serum albumin concentration of ≤3.4 g/dL (AUC= 0.785, P= 0.005) have a diagnostic value in predicting systemic inflammation. A total white blood cell count of ≤5.5x103/μL was also found to be a diagnostic value for predicting systemic inflammation in dogs with parvoviral enteritis (AUC= 0.920, P<0.001). Changes in serum TIBC were not statistically significant in predicting systemic inflammation (P= 0.512). It was concluded that serum iron in particular correlated well with CRP, and when serum iron was used in conjunction with CRP and total WBC, it could be a sensitive marker in assessing systemic inflammation.