In this study, it was aimed to evaluations of the effects of silk suture, polypropylene suture, medical tissue adhesives and skin staples on healing in skin wounds created in rats. A total of 60, 2-months-old winstar albino male rats were used in the study. The subjects were randomly divided into 5 main groups (n=12). Each group was divided into 2 subgroups of 6 units for 7-and 14-day postoperative follow-ups. 6 mg/kg doses xylazin hydrochloride (Rompun of Bayer 23.32 mg/mL) and 85 mg/kg Ketamine hydrochlorur (Ketalar Parke-Davis, 50 mg/mL) were anesthetized with i.m. applications. In the first group (n=12) international standard 0 silk yarn was used, in the second group (n= 12), international standard 0 polypropylene was used, in the third group (n= 12), medical tissue adhesive was used, in the fourth group (n=12) tissue staples were used, the fifth group is the control group (n= 12) Biochemical results showed that the antioxidant property was better in the adhesive and stapler group (P<0.001). Histopathological findings showed that the best wound healing results in incision wound healing were in the stapler group. As a result, it was concluded that staples can be used safely in incisional skin wounds compared to other materials.