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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2005, Cilt 19, Sayı 1, Sayfa(lar) 007-011
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PURIFICATION AND PROKARYOTIC CLONING OF NUCLEOPROTEIN (NP) GENE OF RINDERPEST VIRUS (RPV) RBOK VACCINE STRAIN
Şükrü TONBAK1, Aykut ÖZDARENDELİ1, Mehmet Ziya DOYMAZ2, Yusuf BOLAT1
1Fırat Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Elazığ-TÜRKİYE
2Fırat Üniversitesi, Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ-TÜRKİYE
Keywords: Rinderpest virus, Nucleoprotein gene, Cloning, Plasmid

In this study, it was aimed to expres the NP protein of rinderpest virus (RPV) RBOK vaccine strain in a prokaryotic system. Vero cells were infected with RBOK vaccine strain. Total RNA was isolated from infected cells and reverse transcription was carried out and cDNA of NP gene was obtained. The NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. Nucleoprotein gene of RPV-RBOK vaccine strain was cloned into vector PinPointTM Xa-3 by using the HindIII and KpnI restriction enzyme sites. Recombinant NP protein synthesized by E.coli was purified. To determine the recombinant NP protein which is approximately 80kDa, western blotting was performed.

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