This study was aimed to carry out a research on the effects of aprotinin and methylene blue in the prevention of experimentally performed adhesions in the rat models.

Forty five male rats weighed between 175-200 gr were randomly divided into three groups of 15 rats each. After anesthesia with ether and preparation of the surgical field for aseptic surgery an incision of 3 cm in length was performed along the abdominal midline. The serosal layer of the cecum and the inner surface of abdominal wall corresponding to the cecum were abraded aseptically with a tooth-brush. Groups 1, 2 and 3 rats were given intraperitoneally a single dose of 2.5 ml aprotinin (25000 KIU), methylene blue (1 %) and saline; respectively. The abdominal incision was closed with 3/0 silk suture. Fifteen days after the surgery all rats were killed with overdose administration of ether, their abdominal cavities were exposed and the adhesions occurred were graded on a scale of 0-3.

In group 1, there were 13 rats with grade 0, 1 rat with grade 1 and 1 rat with grade 2 adhesion.

In group 2, there were 7 rats with grade 0, 4 rats with grade 1, 2 rats with grade 2 and 2 rats with grade 3 adhesion.

In the control group there was 1 rat with grade 0, 2 rats with grade 2 and 12 rats with grade 3 adhesion.

The grade of adhesion was significantly higher in groups 1 and 2 compared to the control group (p<0,01).