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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi |
2009, Cilt 23, Sayı 1, Sayfa(lar) 001-004 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Random Amplified Polymorphic DNA (RAPD) Analysis of Escherichia Coli Isolated From Chickens |
Ayşe KILIÇ1, Adile MUZ2, Hasan Basri ERTAŞ2, Gökben ÖZBEY3 |
1Fırat Üniversitesi, Sivrice Meslek Yüksekokulu, Elazığ, TÜRKİYE 2Fırat Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ,TÜRKİYE 3Fırat Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Elazığ, TÜRKİYE |
Keywords: E.coli, RAPD, chicken, PCR |
In this study, 48 E.coli strains isolated from colibacillosis suspicious hens at different poultry
houses were examined. These samples were inoculated on to blood agar and then suspicious
colonies were transferred on to EMB and MacConcey agar. Bacterial growth was observed in 110
samples, 48 (13,6%) of which were identified as Escherichia coli (E.coli). E.coli strains were typed
by randomly amplified polymorphic DNA (RAPD) to detect the genetic differences between at E.coli
strains in this region. Extracted DNA samples from 48 strains of E.coli were amplified by RAPD
primer (COL 1). Nine different band profiles were obtained. These results indicated that there were
great genetic heterogeneity between E.coli strains in this region. RAPD analysis had the highest
discriminatory capacity for typing E.coli isolates. Because of its simplicity and rapidity, RAPD
analysis appears to be a highly valuable tool for studying E.coli molecular epidemiology.
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