In this study, in vitro transcription assay was performed using DNA templates for matrix (M) gene and nucleoprotein (NP) gene of rinderpest (RPV) RBOK vaccine strain. All kind of in vitro transcription of DNA into RNA is carried out with bacteriophage-encoded DNA-dependent RNA polymerases. The RNA polymerases recognise the spesific DNA region called promotor to transcribe of DNA into RNA. Thus, it was cloned both M and NP genes of RPV RBOK vaccine strain into pCI-neo and pGEM -3Zf (-) were cloned using vector containing T7 promotor site. After in vitro trancription assay, the RNA transcripts were obtained and visuliazed on agorose gel.