In the present study, expression of nucleoprotein (NP) gene of rinderpest virus (RPV) RBOK vaccine strain in eucaryotic cells was performed. The NP gene of RPV-RBOK vaccine strain was cloned into a pGEM-3Zf (-) vektor. The recombinant plasmid was cut with NheI and SalI enzymes to obtain the NP gene. Then, the NP gene was placed into pCI-neo mammalian expression vektor. The resulting recombinant plasmid was named as pSV-NP. pSV-NP was transiently transfected into Vero cells. The presence of the NP proteins was analaysed by Western Immunoblotting demonstrating that Vero cells express a 60 kDa protein reacting with anti-NP antibody.