In this study, the inhibition effects of EDTA, p-CMBA, NEM on arginase activity in sheep kidney tissue were studied. Arginase activity was spectrophotometrically measured using the Tiyosemicarbazid- Diacetylmonoksim-Urea Method. Protein was determined by the method of Lowry. The kidney tissues of sheep in the present study were obtained from the Elkas sloughterhause in Elazıg. This study was carried out using 20 sample of kidney tissue. In conclusion, arginase activity in sheep kidney tissue was determined to be inhibited competative inhibition by EDTA but to be inhibited noncompetative inhibition by NEM, p-CMBA.