In this study, it was aimed to present the diversity in teat skin lesions and their effects on mastitis in dairy cows. The animal material induced 30 Holstein cows on lactation and with teat lesions. Samples of milk and swabs of teat lesions were cultured for isolation of bacteria. After this process, California Mastitis Test (CMT) was performed. Milk samples and swabs of lesions were cultured for bacterial analysis. Antibiotic sensitivity test was performed and the isolates were confirmed by polimerase chain reaction (PCR). Lesions were cuts (10%), chup (33.33%), eczema (23.33%), papillomatous (23.33%) and hyperkeratosis (10%). The teat lesions were less identified at short teat type than medium and long (P<0.01). The teats were classified as a cylindrical (53.3%), funnel (33.3%) and smooth (13.3%) (P<0.01). Rates of bacterial isolation form milk samples were 75% in cylindrical teat type, 70% funnel teat type and 50% smooth teat type (P>0.05). The most common bacteria was Staphylococcus spp at teat lesions and most of them was KNS. Streptococcus spp was also isolated from the same samples. It was determined that the most proliferate bacteria was KNS (60%) both CMT positive and negative. Bacteria was detected in 70% of the milk samples taken from teats with lesions. Antibiotic sensitivity was shown similar in both milk samples and teat lesion samples. As a result, attention should be paid to the teat lesions that cause predisposition to mastitis, and also structural traits of the teats should be taken into consideration for prevention from teat skin lesions.