The increase in the demand for fish has led to intensive aquaculture practices which has also ushered in a manifestation and emergence of new fish diseases and reoccurrence of old ones. The advocacy for alternative therapy to drugs using natural and environmental friendly materials necessitated this study. The antimicrobial properties of Tridax procumbens extracts were tested against pathogenic fish bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aereus, Aeromonashydrophilia, and Listeria monocytogenes). Bacteria were cultured, minimum inhibitory concentration (MIC) and zone of inhibition (ZI) were carried out using standard methods. One hundred and eight (108) Hetrobranchus bidorsalis juveniles were fed T. procumbens based diets (40% crude protein) two times daily in a completely randomized designed for 54 days. Data obtained were analyzed using descriptive statistics and ANOVA at α0.05. The results shows that T. procumbens extract contained alkaloids, flavonoids, tannins, steroids and carptenoids while saponin and terpnoids were not detected in the extract. Effective MIC was 40mg/ml and ZI ranged from 12.85±0.39 to 14.20±0.74 mm. Pathogens were sensitive to the T. procumbens extract except for L. monocyogenes. The TVC and TCC were significantly reduced in the treated group. The results revealed T. procumbens leaves have antimicrobial properties which may had shown its potential utilization in aquaculture. It also indicated that T. procumbens have antimicrobial activity against tested organisms and could be used fish health management.