Extended spectrum beta lactamases (ESBL) produced by Gram negative bacteria confer resistance to all penicillins, 1st-4th generation cephalosporins and monobactams. In the present study, existence of ESBL producing E. coli isolates and frequency of the most prevalent GSBL genes in such E. coli isolates were investigated in the healthy calves in Burdur city of Turkey. A 10% suspension was prepared from each faeces (n=150) in peptone buffered water and spread on E. coli / coliform selective agar supplemented with cefotaxime and ceftazidime. The colonies appeared on the media were identified as E. coli by standard methods and genetic confirmation for E. coli was performed by polymerase chain reaction (PCR). Then ESBL production of E. coli isolates were confirmed by combined disc method and the common ESBL genes Cefotaximase-Munich (CTX-M), Temoneira (TEM) and Sulfhydryl variable (SHV) were investigated by PCR. Finally, susceptibilities of the ESBL producing E. coli isolates to several beta lactams and other classes of antibiotics were investigated by agar disc diffusion test. Forty four E. coli isolates were isolated from selective media but 36 of them were confirmed as ESBL producer. CTX-M (group 1) genes were found in all isolates but SHV gene was not detected in the isolates. ESBL producing E. coli isolates were found resistant at high rate against to beta lactam group and other groups of antibiotics. Consequently, this study showed the presence of ESBL producing E. coli in the healthy calves in Burdur city of Turkey and also prevalence of types of ESBL genes in ESBL producing E. coli isolates.