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Fırat University Journal of Health Sciences (Veterinary) |
2018, Cilt 32, Sayı 3, Sayfa(lar) 179-183 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Protective Effect of Naringin on Methotrexate-Induced Testicular Apoptosis and Autophagy Via Reducing Oxidative Stress in Male Rats |
Emrah Hicazi AKSU1, Fatih Mehmet KANDEMİR2, Sefa KÜÇÜKLER2 |
1University of Atatürk, Faculty of Veterinary, Department of Reproduction and Artificial Insemination, Erzurum, TURKEY 2University of Atatürk, Faculty of Veterinary, Department of Biochemistry, Erzurum, TURKEY |
Keywords: Reproductive toxicity, methotrexate, naringine, apoptosis, autophagy |
In this study the possible protective effect of naringin (NRG) on methotrexate (MTX)–induced damage on testes of rats was investigated. A total of 35 male rats were used as materials.
Animals were divided into 5 groups, 7 rats in each; Group 1 (Control): A single dose injection of physiological saline was administered intraperitoneally on the first day. Group 2 (NRG 100): NRG (100 mg/kg b.w./ day) was given orally for 7 days. Group 3 (MTX): Single dose of 20 mg/kg of MTX was given intraperitoneally on the first day. Group 4 (NRG 50+MTX) 50 mg/kg/day NRG was given for 7 days after a single intraperitoneal injection of 20 mg/kg MTX. Group 5 (NRG 100+MTX): 100 mg/kg/day NRG was given for 7 days after a single intraperitoneal injection of 20 mg/kg MTX. As a results, MTX increased to malondialdehyde (MDA) levels and decreased glutathione (GSH) level and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities when compared to control group. On the other hand, NRG (100 mg/kg) treatment caused decreasing the MDA levels and increa the GSH levels and SOD, CAT, and GSH-Px activities when compared to MTX alone group. NRG 50 mg/kg treatment did not increase SOD activity. Also, MTX treatment increased caspase-3 and B-cell lymphoma 3 protein (BCL-3) expression levels in testicular tissue when compared to control group. However, NRG (both at the dose of 50 mg/kg and 100 mg/kg) treatment lightened significantly the testis caspase-3 and BCL-3 expression levels when compared to MTX alone group. In conclusion, NRG (especially at the dose of 100 mg/kg) treatment decreased MTX-induced testicular toxicity in male rats. |
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