This study was conducted to determine which level of noise causes oxidative stress and to investigate the effect of this stress on sperm quality. In the study, 28 male Wistar-albino rats of 2–3 months were equally divided into 4 groups. The first group was used as control. The second, the third, and the fourth groups were exposed to low (60 dB), medium (80 dB) and high (100 dB) intensity noise, respectively, and 8.000 Hertz frequency for 12 hours hours every night for 60 nights. After 60 days, blood and semen samples were taken and examined. Increased levels of malondialdehyde (MDA), a marker of lipid peroxidation, due to high-level noise, increased catalase (CAT) activity, and increased glutathione peroxidase (GSH-Px) activity indicate the occurence of oxidative stress. In addition, abnormal spermatozoon rate was increased. This results shows that the sperm quality decreased. CAT levels and GSH-Px activity were also increased at low and moderate noise levels. Therefore, we conclude that oxidative stress caused by high-level noise application may lead to lipid peroxidation in particular resulting in various damages to the cell level.