This study was aimed to determine the level aflatoxin M1 (AFM1) level in raw cow and Anatolian buffalo milk collected from Diyarbakır city. For this purpose, a total of 84 milk samples, 42 raw cow’s milk and 42 raw Anatolian buffalo milk collected from small-scale family enterprises in Diyarbakır, were used as material. The level of AFM1 in milk samples were determined by ELISA method. In the analyzed raw cow milk samples, the amount of AFM1 was found to be between 5 and 100 ng/L in 36 (85.7%) and the average concentration was 24.80±15.89 ng/L, and it was higher than 100 ng/L in 4 (9.5%). In buffalo milks, AFM1 was found to be with average concentration of 10.22±4.25 ng/L between 5 and 50 ng/L in 16 (38.1%) samples, while it was below concentration of 50 ng/L in all samples (100%). AFM1 average concentration in cow's milk was higher than that in buffalo milk (P<0.05). The concentration of AFM1 in 7 (16,6%) of cow's milk was higher than the maximum tolerance limit (50 ng/L) accepted by the Turkish Food Codex and the European Union criteria. The results of this study showed that Anatolian buffalo milk is less risky for AFM1 than cow milk and cow milk continues to be an important source for AFM1.