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Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
2009, Cilt 23, Sayı 2, Sayfa(lar) 073-078
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Effect of L- Lysine and L- Ornithine on Buffalo Liver and Kidney Tissue Arginase
Mehtap ÖZÇELİK1, Necmi ÖZDEMİR2
1Veteriner Kontrol ve Araştırma Enstitüsü, Elazığ, TÜRKİYE
2Fırat Üniversitesi, Veteriner Fakültesi Biyokimya Anabilim Dalı, Elazığ, TÜRKİYE
Keywords: Arginase, Lysine, Ornithine

In this study, at different concentrations, L- ornithine and L- lysine were demonstrated to be inhibited arginase activity levels in buffalo liver and kidney tissue. At 20 mM concentration, L- ornithine inhibited liver tissue arginase and kidney tissue arginase by 60 and 39% respectively. Whereas at 80 mM inhibitory effect of L- ornithine were found 89 % and 86 % in liver and kidney tissue arginases, respectively. On the other hand, L- lysine at 20 mM concentration was determined to inhibit liver and kidney tissue arginase activity levels by 47 % and 41 %, respectively. When effect of higher concentration (80 mM) of L- lysine was examined, it was determined that inhibition of liver and kidney tissue arginases were 81 % and 79 %, respectively. With different substrates, liver tissue was saturated after 20 mM L- arginine whereas kidney tissue was saturated after 50 mM of L- arginine. Based on these results, when 50 mM L- ornithine and L- lysine were added at different substrate concentrations, Km of buffalo arginase was determined to be different at liver ( 2.4 mM) and kidney (8 mM) tissues.

In conclusion, arginase activity levels in liver and kidney tissue were determined to be inhibited differently. Arginase activity in Buffalo liver and kidney tissue arginases were demonstrated to be inhibited via mixed inhibition (competetive- non competetive) by L- ornithine and L- lysine.


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